News

 

Information

General information, events and/or links.

 

 

EV literature (RSS feed from PubMed)

Search terms: exosomes OR "extracellular vesicles" OR microvesicles OR microparticles. Direct link to the PubMed search here.

Reply: Exosomes are Comparable to Source Adipose Stem Cells in Fat Graft Retention with Up-Regulating Early Inflammation and Angiogenesis.

-

Icon for Wolters Kluwer Related Articles

Reply: Exosomes are Comparable to Source Adipose Stem Cells in Fat Graft Retention with Up-Regulating Early Inflammation and Angiogenesis.

Plast Reconstr Surg. 2020 Aug;146(2):232e-233e

Authors: Chen B, Guo L

PMID: 32740620 [PubMed - as supplied by publisher]

Exosomes Are Comparable to Source Adipose Stem Cells in Fat Graft Retention with Up-Regulating Early Inflammation and Angiogenesis.

-

Icon for Wolters Kluwer Related Articles

Exosomes Are Comparable to Source Adipose Stem Cells in Fat Graft Retention with Up-Regulating Early Inflammation and Angiogenesis.

Plast Reconstr Surg. 2020 Aug;146(2):232e

Authors: Boriani F, Perut F

PMID: 32740619 [PubMed - as supplied by publisher]

Injectable PLGA-coated ropivacaine produces a long-lasting analgesic effect on incisional pain and neuropathic pain.

-

Icon for Elsevier Science Related Articles

Injectable PLGA-coated ropivacaine produces a long-lasting analgesic effect on incisional pain and neuropathic pain.

J Pain. 2020 Jul 30;:

Authors: Tian X, Zhu H, Du S, Zhang XQ, Lin F, Ji F, Tsou YH, Li Z, Feng Y, Ticehurst K, Hannaford S, Xu X, Tao YX

Abstract
The management of persistent postsurgical pain and neuropathic pain remains a challenge in the clinic. Local anesthetics have been widely used as simple and effective treatment for these two disorders, but the duration of their analgesic effect is short. We here reported a new poly lactic-co-glycolic acid (PLGA)-coated ropivacaine that was continuously released in vitro for at least 6 days. Peri-sciatic nerve injection of the PLGA-coated ropivacaine attenuated paw incision-induced mechanical allodynia and heat hyperalgesia during the incisional pain period, and spared nerve injury-induced mechanical and cold allodynia for at least 7 days post-injection. This effect was dose-dependent. Peri-sciatic nerve injection of the PLGA-coated ropivacaine did not produce detectable inflammation, tissue irritation, or damage in the sciatic nerve and surrounding muscles at the injected site, dorsal root ganglion, spinal cord or brain cortex, although the scores for grasping reflex were mildly and transiently reduced in the higher dosage-treated groups. Perspective: Given that PLGA is an FDA-approved medical material, and that ropivacaine is used currently in clinical practice, the injectable PLGA-coated ropivacaine represents a new and highly promising avenue in the management of postsurgical pain and neuropathic pain.

PMID: 32739615 [PubMed - as supplied by publisher]

Macrophage-derived extracellular vesicles regulate concanavalin A-induced hepatitis by suppressing macrophage cytokine production.

-

Icon for Elsevier Science Related Articles

Macrophage-derived extracellular vesicles regulate concanavalin A-induced hepatitis by suppressing macrophage cytokine production.

Toxicology. 2020 Jul 30;:152544

Authors: Kawata R, Oda S, Koya Y, Kajiyama H, Yokoi T

Abstract
Acute liver failure is a clinical syndrome of severe hepatic dysfunction. Immune cells play an important role in acute liver failure. In recent years, the immunoregulatory function of extracellular vesicles (EVs) has been reported; therefore, it is inferred that EVs play a role in immune-mediated hepatitis. In this study, we investigated the immunoregulatory function of EVs in concanavalin A (Con A)-induced hepatitis. The mouse model was prepared by a single intravenous administration of 15 mg/kg Con A, in which there was a significant increase in the serum EVs number. In an in vitro study, the number of secreted EVs was also significantly increased in Con A-treated RAW264.7 cells, a mouse macrophage cell line, but not in Hepa1-6 cells, a mouse hepatoma cell line. In an in vitro EVs treatment study, EVs from Con A-treated mouse serum and Con A-treated RAW264.7 cells suppressed inflammatory cytokine production in Con A-stimulated RAW264.7 cells. miRNA sequencing analysis showed that the expression of mmu-miR-122-5p and mmu-miR-148a-3p was commonly increased in these EVs and EVs-treated cells. The pathways enriched in the predicted miRNA target genes included inflammatory response pathways. The mRNA levels of the target genes in these pathways (mitogen-activated protein kinase, phosphoinositide 3-kinase/Akt and Rho/Rho-associated coiled-coil containing protein kinase pathways) were decreased in the EVs-treated cells. In an in vivo RNA interference study, the knockdown of liver RAB27A, an EVs secretion regulator, significantly exacerbated Con A-induced hepatitis. These data suggest that macrophage-derived EVs play an important role in Con A-induced hepatitis through immunoregulation.

PMID: 32739513 [PubMed - as supplied by publisher]

Do Not Flick or Drop Off-label Use Plastic Syringes in Handling Therapeutic Proteins Before Administration.

-

Icon for Elsevier Science Related Articles

Do Not Flick or Drop Off-label Use Plastic Syringes in Handling Therapeutic Proteins Before Administration.

Int J Pharm. 2020 Jul 30;:119704

Authors: Ah Kim N, Jun Kim D, Hoon Jeong S

Abstract
This study investigates the formation of subvisible particles formed by external stresses produced such as flicking and dropping syringes. Flow imaging was used to visualize and quantify microparticles from 1 μm to over 25 μm as a result of mishandling. Microparticles increased in the presence of silicone oil that was present in syringes. Thus, silicone oil in syringes may affect the activity of therapeutic proteins being injected. Present data showed detailed and differentiated morphologies of proteinaceous particles, silicone oil, air bubbles, and plastic debris in mishandled syringes. In some cases, the presence of bisphenol A in syringes was detected by FT-IR. Disposable plastic syringes were evaluated and showed differences in their content of silicone oil. Syringes that contain 0.45 μm filters inside the needle cap as well as silicone oil-free syringes release proteinaceous subvisible particles after mechanical stress. These stress-generated particles can be delivered to patients, compromising patient care.

PMID: 32739384 [PubMed - as supplied by publisher]

Astrocyte regional diversity in ALS includes distinct aberrant phenotypes with common and causal pathological processes.

-

Icon for Elsevier Science Related Articles

Astrocyte regional diversity in ALS includes distinct aberrant phenotypes with common and causal pathological processes.

Exp Cell Res. 2020 Jul 30;:112209

Authors: Gomes C, Sequeira C, Barbosa M, Cunha C, Vaz AR, Brites D

Abstract
Astrocytes are major contributors of motor neuron (MN) degeneration in amyotrophic lateral sclerosis (ALS). We investigated whether regional and cell maturation differences influence ALS astrocyte malfunction. Spinal and cortical astrocytes from SOD1G93A (mSOD1) 7-day-old mice were cultured for 5 and 13 days in vitro (DIV). Astrocyte aberrancies predominated in 13DIV cells with region specificity. 13DIV cortical mSOD1 astrocytes showed early morphological changes and a predominant reactive and inflammatory phenotype, while repressed proteins and genes were found in spinal ones. Inflammatory-associated miRNAs, e.g. miR-155/miR-21/miR-146a, were downregulated in the first and upregulated in the later. Interestingly, depleted miR-155/miR-21/miR-146a in small extracellular vesicles (sEVs/exosomes) was a common pathological feature. Cortical mSOD1 astrocytes induced late apoptosis and kinesin-1 downregulation in mSOD1 NSC-34 MNs, whereas spinal cells upregulated dynein, while decreased nNOS and synaptic-related genes. Both regional-distinct mSOD1 astrocytes enhanced iNOS gene expression in mSOD1 MNs. We provide information on the potential contribution of astrocytes to ALS bulbar-vs. spinal-onset pathology, local influence on neuronal dysfunction and their shared miRNA-depleted exosome trafficking. These causal and common features may have potential therapeutic implications in ALS. Future studies should clarify if astrocyte-derived sEVs are active players in ALS-related neuroinflammation and glial activation.

PMID: 32739211 [PubMed - as supplied by publisher]

Corrigendum to "Exosomes derived from microRNA-30b-3p-overexpressing mesenchymal stem cells protect against lipopolysaccharide-induced acute lung injury by inhibiting SAA3" [Exp. Cell. Res. 383 (2019) 111,454].

-

Icon for Elsevier Science Related Articles

Corrigendum to "Exosomes derived from microRNA-30b-3p-overexpressing mesenchymal stem cells protect against lipopolysaccharide-induced acute lung injury by inhibiting SAA3" [Exp. Cell. Res. 383 (2019) 111,454].

Exp Cell Res. 2020 Jul 29;:112160

Authors: Yi X, Wei X, Lv H, An Y, Li L, Lu P, Yang Y, Zhang Q, Yi H, Chen G

PMID: 32739028 [PubMed - as supplied by publisher]

 

Previous page: Relevant links  Next page: EVents