News

Aug 23, 2016

Join the SELECTBIO Extracellular Vesicles Virtual Event made available from September 21st, and hear leading researchers talk about topics within EV biology and therapeutic potential!

 

Information

General information, events and/or links.

 

 

Thermo Fisher Scientific

Group information, relevant EV research and instrumentation

Group name

Thermo Fisher Scientific, Life Sciences Solutions, Synthetic Biology, Norway

Affilitation

 

Ullernchausséen 52, 0379 Oslo, Norway

Link to homepage: www.thermofisher.com

 

 

Name

E-mail

Phone

Steering group member

Axl Neurauter

Axl.neurauter@thermofisher.com

+47 90 18 93 65

Deputy representative

Malin Karlsson

Malin.karlsson@thermofisher.com

+47 97 47 54 47

Group members

Bente Kierulf

Bente.kierulf@thermofisher.com

+47 93 40 13 55

Ketil Winther Pedersen

Ketil.pedersen@thermofisher.com

+47 91 18 14 78

Erlend Ragnhildstveit

Erlend.ragnhildstveit@thermofisher.com

+47 95 40 34 50

 

Scientific background/relevant EV research:

Life Technologies Inc is a biotech company delivering products and services into life science research, diagnostics and cell based therapy. The short term focus for our exosome/microvesicle initiative is development of reagents and methods for generic and specific isolation and analysis of exosomes/microvesicles with in life science research. The longer term goal is to explore the opportunities for exosome/microvesicle based diagnostics. 

 

In 2012/Q1 2013 Life Technologies has launched 7 products for exosome/microvesicle research:

-    products for generic isolation of exosomes/microvesicles from cell culture and human serum

-    products for specific isolation/detection of exosome/microvesicle sub-populations from cell  
culture (CD63 and Streptavidin magnetic beads)

-    one product for isolation of nucleic acid (and protein) from exosomes/microvesicles (Trizol)

-    two products for isolation of proteins from exosomes/microvesicles (Prot A/G magnetic beads)

 

Ongoing research includes standardization and expand the product portfolio for specific isolation/detection of exosome/microvesicle sub-populations targeting molecules such as CD9 and CD81. Included in standardization work is:

-    optimized production of exosome enriched cell culture supernatants

-    methods for flow analysis of exosomes

In additions, analysis methods such as EM and western have been established better characterize the exosomes/microvesicles and their content, while methods such as MS, qRT-PCR and sequencing are being explored.

 

Future work will include transition to body fluids such as serum/plasma and urine as sources for exosome/microvesicle isolation/detection.  

 

Instrumentation (relevant for EV research):

  • NanoSight (LM10)
  • Western blotting (gel, iBlot = dry blotting system)
  • ChemiDoc MP Imager (fluorescence and bright light gel/blot Imager)
  • BD LSR II flow instrument
  • Floid (fluorescence microscope with very easy user interface and image capture capabilities)
  • Fluorescence microscope (Nikon)
  • Qbit and NanoDrop (very low volume protein and NA measurements)
  • EM expertise (do not have instrument but buy access at UiO - IMBV, Prof. Norbert Roos)
  • Beckman J2-21 Centrifuge (fix angle); JA-20 rotor 48400xg 400 ml, JA-10 rotor 17700xg 3000 ml
  • Applied Biosystems 7500 real time PCR system
  • Wallac Victor2 1420 Multicounter (delayed fluorescence platform)
  • Biotek Synergy 4 (spectrophotometer)

 

 

Date 

August 21st, 2015